BIOREADER®-7000-V

Non-fluoro applications in 384 well –6 Well plates and Petridishes.

BIOREADER®-7000-V
Non-fluoro applications in 384 well –6 Well plates and Petridishes.

The BIOREADER®-7000-V is equipped with a fully automatic fixed lens changer.

Flexibility

The Bioreader® hardware may be upgraded on site. Brickstone principle.

Independence

Bioreader® operates with all commonly used Elispot/Fluorospot/Plaque/Fluoro-Plaque/CFU assays.
You can choose among all major assay manufactures.

Features
  • Patented dual telecentric illumination
  • ’EazyReader® software generation 20’
  • Front loader with automatic door
  • Fold lens changer with high definition fix focus lenses
  • Automated focus, illumination, lens changer are measure protocol controlled and locked
  • Standard 5 MPixel camera (up to 42 MPixel available)
  • Optional 21 cfr part 11 software module (GAMP IQ/OQ/PQ/DQ/FAT/SAT)
  • DELL PC with 3 years NBD on-site service and warranty (Office/Windows 10)
  • Loudspeakers
  • For easy and powerful usage, incl. Q.M. package
  • Excel/Word/PowerPoint and LIMS export capabilities
  • Interactive content specific video training program
  • ‘Clinical routine’ and ‘research’ user interface
  • For ‘phantom spot suppression’, better spot separation and cytokine quantification
Software

biocreader_7000_v_software.png

Eazyreader® software

  • Innovative Eazyreader® software combines ‘easy of use’ and versatility and flexibility
  • Optimized applications for commonly used operations like ‘routine scan’
  • QC presents only the icons you requires for this job
  • Scan, analyze and overlay live time
  • creates up to 7 images for each well simultaneously during te scan already
  • ‘Profiling’ help to create ‘user independent’ measure protocols for Elispot beginners and references fro experts
  • Verified in collaborative studies
  • Export options including reports with all scalable images and results in one file
  • Customer specific report templates
  • Video clips and content specific help files
  • Qualified installation and training on side with each Bioreader®
  • On side support and internet remote support
  • ‘Classified’ measure protocols ‘history’ tracking and comparison tools
  • User specific projects, designs, protocols and studies prevent from mixup
  • More accurate ‘cytokine quantification’ based on the patented ‘photometric’ dual illumination system
  • CE EMV certificate
  • 21 cfr part 11 module
  • Full automation optional with ‘feeding system’

 

Dimension

width
45 cm
height
60 cm
depth
35 cm

Weight

weight
26 kg
The BIOREADER®-7000-V can also use with the follow applications:
Plaque assay: very bright plaques in 24 well plate Icon

Plaque assay: very bright plaques in 24 well plate

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

Foci assay: total well view Icon

Foci assay: total well view

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed."

[https://en.wikipedia.org/wiki/Virus_quantification]

Elispot in 384 multifilterplates Icon

Elispot in 384 multifilterplates

Small volume, high throughput encymatic Elispot.

"The enzyme-linked immune absorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell."

[https://en.wikipedia.org/wiki/ELISpot]

Elispot single enzymatic Icon

Elispot single enzymatic

Blue/red/green/silver substrat Elispot. Detection antibody, enzyme-conjugate and precipitating substrate.

Dual Elispot enzymatic 1-2 colors Icon

Dual Elispot enzymatic 1-2 colors

blue/red substrate and mixed color violet

Unstained cells Icon

Unstained cells

Alveolar epithelial cells (AEC) Icon

Alveolar epithelial cells (AEC)

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

AEC graylevels Icon

AEC graylevels

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

MCMV (Mouse Cytomegalovirus) unstained Icon

MCMV (Mouse Cytomegalovirus) unstained

HCMV (Human cytomegalovirus): unstained Icon

HCMV (Human cytomegalovirus): unstained

"The glycoprotein gO (UL74) of human cytomegalovirus (HCMV) forms a complex with gH/gL. Virus mutants with a deletion of gO show a defect in secondary envelopment with the consequence that virus spread is restricted to a cell-associated pathway."

[https://www.ncbi.nlm.nih.gov/pubmed/20181688]

NK92 cells natural killer cells (human cell line) same samples microwell plate. Icon

NK92 cells natural killer cells (human cell line) same samples microwell plate.

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis."

[https://en.wikipedia.org/wiki/Natural_killer_cell]

Foci assay: Very large Foci Icon

Foci assay: Very large Foci

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed."

[https://en.wikipedia.org/wiki/Virus_quantification]

Plaque assay extremely large spots Icon

Plaque assay extremely large spots

"The TCID50 (Median Tissue Culture Infectious Dose) is one of the methods used when verifying viral titer. TCID50 signifies the concentration at which 50% of the cells are infected when a test tube or well plate upon which cells have been cultured is inoculated with a diluted solution of viral fluid."

[https://www.zeomic.co.jp/en/glossary/virus/71]

CV Plaque assay.Example with large and fuzzy plaques Icon

CV Plaque assay.Example with large and fuzzy plaques

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

TCID50 viral assay Icon

TCID50 viral assay

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

Various media/strain combinations on petridishes Icon

Various media/strain combinations on petridishes

Various colonies in 6 well plates and on 35 mm - 100 mm petridishes Icon

Various colonies in 6 well plates and on 35 mm - 100 mm petridishes

Dual Transgene assay Icon

Dual Transgene assay

Transgen:

A segment of DNA introduced to some other organism.

See [https://en.wikipedia.org/wiki/Transgene]

Organoids Icon

Organoids

"An organoid is a miniaturized and simplified version of an organ produced in vitro in three dimensions that shows realistic micro-anatomy. The technique for growing organoids has rapidly improved since the early 2010s."

[https://en.wikipedia.org/wiki/Organoid]

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 568 labelled secondary antibodies Icon

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 568 labelled secondary antibodies

"Marek's disease is a highly contagious viral neoplastic disease in chickens. The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes. Viruses related to MDV appear to be benign and can be used as vaccine strains to prevent Marek's disease."

[https://en.wikipedia.org/wiki/Marek's_disease]

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies Icon

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies

"Marek's disease is a highly contagious viral neoplastic disease in chickens. The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes. Viruses related to MDV appear to be benign and can be used as vaccine strains to prevent Marek's disease."

[https://en.wikipedia.org/wiki/Marek's_disease]

Neutralization assay: accepted plaques are surrounded Icon

Neutralization assay: accepted plaques are surrounded

"The plaque reduction neutralization test is used to quantify the titer of neutralizing antibodies for a virus. The serum sample or solution of antibodies to be tested is diluted and mixed with a viral suspension."

[https://en.wikipedia.org/wiki/Plaque_reduction_neutralization_test]

Neutralization assay Icon

Neutralization assay

"The plaque reduction neutralization test is used to quantify the titer of neutralizing antibodies for a virus. The serum sample or solution of antibodies to be tested is diluted and mixed with a viral suspension."

[https://en.wikipedia.org/wiki/Plaque_reduction_neutralization_test]

AEC color Icon

AEC color

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

loading