BIOREADER®-7000-F

Fluoro Elispot/Plaque + Enzymatic Readers for 96 wells with innovative power LED fluoro excitation.

BIOREADER®-7000-F
Fluoro Elispot/Plaque + Enzymatic Readers for 96 wells with innovative power LED fluoro excitation.

The BIOREADER®-7000-F is equipped with a fluorescent light source, and Olympus fluorescence filter slots. This allows you to evaluate verified enzymatic Elispot or Fluorospot counting.

This BIOREADER®-7000-F applies up to 10  Standard-Olympus®-flouro-filter-cubes automatically..

These Olympus® or compatible Filter cubes maybe replaces even -on site-.

Model variants    Filter cubes installiert       Maximal number of filtercubes

Alpha                                           2                                               6

Beta                                             3                                               6

Gamma                                       4                                              10

Delta                                            6                                              10

Flexibility

The Bioreader® hardware may be upgraded on site. Brickstone principle.

Independence

Bioreader® operates with all commonly used Elispot/Fluorospot/Plaque/Fluoro-Plaque/CFU assays.
You can choose among all major assay manufactures.

Features
  • 6-10 times fluoro-filter changer: for Standard Olympus cubes on all Bioreader-F models.
  • New high sensitive, but low noise camera technology with optimal lens glasses for high contrasted images.
  • Upgrade option: from model alpha to delta (max 10 active filters cubes) 
  • 3D spot-volume cytokine quantification: provides more sensitive stimulation response results.
  • Bioreader® -F 7000 is an affordable model for fluoro and enzymatic Elispot and Plaque/Foci counting in 384/96 well plates. 
  • Single click export to PDF, Microsoft Office® applications.
  • Counting is significantly faster and more precise regarding positioning.
  • Upgradable: to 21 cfr part 11 bases software package.
  • Front loader, automatic door
  • Dual tele-centric illumination
  • Loud speaker and interactive training program
  • Excel/Word/Power Point and LIMS export capabilities
  • 'routine' and 'research' user interface
  • High contrast/low noise Cameras 5-41 MPixel available
Software

biocreader_7000_f_software.png

Eazyreader® software

  • Innovative Eazyreader® software combines ‘easy of use’ and versatility and flexibility
  • Optimized applications for commonly used operations like ‘routine scan’
  • QC presents only the icons you requires for this job
  • Scan, analyze and overlay live time
  • creates up to 7 images for each well simultaneously during te scan already
  • ‘Profiling’ help to create ‘user independent’ measure protocols for Elispot beginners and references fro experts
  • Verified in collaborative studies
  • Export options including reports with all scalable images and results in one file
  • Customer specific report templates
  • Video clips and content specific help files
  • Qualified installation and training on side with each Bioreader®
  • On side support and internet remote support
  • ‘Classified’ measure protocols ‘history’ tracking and comparison tools
  • User specific projects, designs, protocols and studies prevent from mixup
  • More accurate ‘cytokine quantification’ based on the patented ‘photometric’ dual illumination system
  • CE EMV certificate
  • 21 cfr part 11 module
  • Full automation optional with ‘feeding system’

 

Dimension

width
60 cm
height
60 cm
depth
45 cm

Weight

weight
35 kg
The BIOREADER®-7000-F can also use with the follow applications:
Fluorospot 1-4 colors Icon

Fluorospot 1-4 colors

"The FluoroSpot measures the secretion of several cytokines by the same cell using a cytokine-specific fluorochromic antibody studied."

Why use Fluorospot

•Enzymatic Elispot labeling
•allows to label 1-2 cytokines per cell synchronous.
•If you are interested to see ‘double secreting’ cells the stain of two cells in neighborhood may overlap. So, It is not clear if two cells are overlapping or one cells is really secreting two different cytokines ( see below left)

‘Fluorospot’ labeling
Allows to label a (theoretical unlimited number different cytokines) Because excitation for each cytokine is applied -one after the other-, decisions for double secreting cells is clearly.
One Image is acquired for each cytokine!

Currently Fluorospot assays with 1-4 Fluorophores are on the market.

Fluorospot offer's significant advantages over colorimetric formats, particularly in the areas of multiplexing and automated spot detection.

Moreover, as spot development is not enzymatic, signal intensity is directly proportional to the amount of analyte within.

[https://fr.wikipedia.org/wiki/FluoroSpot]

Elispot in 384 multifilterplates Icon

Elispot in 384 multifilterplates

Small volume, high throughput encymatic Elispot.

"The enzyme-linked immune absorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell."

Why Elispot?

•The cytokine Elispot assay is designed to enumerate cytokine secreting cells in single cell suspensions of lymphoid tissue, CNS tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMC).

•The assay has the advantage of detecting only activated/memory T cells and the cytokine release can be detected at the single cell level, allowing direct determination of T cell frequencies.

The ELISPOT assay is an effective tool to enumerate antigen-specific T cells in the circulation of immunized humans and animals at much lower frequencies than possible with other currently available methods.

The ELISPOT assay has proven to be a sensitive and unique system to follow disease progression in human individuals or animals. Several studies have indicated that alterations in the frequency of cytokine pc in different compartments of the body adequately reflect changes in immune function.

The ELISPOT assay may be used to determine effects of drugs, chemicals or other compounds on cytokine secretion in vitro, thereby providing data on their putative modulatory effects on immune function in vivo

The ELISPOT assay is currently being used increasingly for the quantitative assessment of peptide reactive T lymphocytes from PBMC in infectious disease (3, 9) or in the course of vaccination trials aimed at the induction of tumor-specific T cells in patients with cancer.

Elispot may be used for Diagnosis of genetic defects,Allergic diseases,Autoimmune diseases,Transplantation,Cancer research,Acute inflammation,Acute infectious diseases and septic shock.•

[https://en.wikipedia.org/wiki/ELISpot]

Elispot single enzymatic Icon

Elispot single enzymatic

Blue/red/green/silver substrat Elispot. Detection antibody, enzyme-conjugate and precipitating substrate.

 

Dual Elispot enzymatic 1-2 colors Icon

Dual Elispot enzymatic 1-2 colors

blue/red substrate and mixed color violet

RFP-expressed virus infected Icon

RFP-expressed virus infected

"Using DNA recombinant technology, scientists combine the GFP gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell."

[https://embryo.asu.edu/pages/green-fluorescent-protein but with RFP instead of GFP]

Unstained cells Icon

Unstained cells

Alveolar epithelial cells (AEC) Icon

Alveolar epithelial cells (AEC)

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

AEC graylevels Icon

AEC graylevels

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

MCMV (Murine Cytomegalovirus) greenfluoro remission Icon

MCMV (Murine Cytomegalovirus) greenfluoro remission

"Cytomegalovirus (CMV) (from the Greek cyto-, "cell," and megalo-, "large") is a genus of viruses in the order Herpesvirales."

[https://en.wikipedia.org/wiki/Cytomegalovirus]

MCMV (Murine Cytomegalovirus) red fluoro remission. Icon

MCMV (Murine Cytomegalovirus) red fluoro remission.

"Cytomegalovirus (CMV) (from the Greek cyto-, "cell," and megalo-, "large") is a genus of viruses in the order Herpesvirales."

[https://en.wikipedia.org/wiki/Cytomegalovirus]

MCMV (Mouse Cytomegalovirus) unstained Icon

MCMV (Mouse Cytomegalovirus) unstained

HCMV (Human cytomegalovirus): unstained Icon

HCMV (Human cytomegalovirus): unstained

"The glycoprotein gO (UL74) of human cytomegalovirus (HCMV) forms a complex with gH/gL. Virus mutants with a deletion of gO show a defect in secondary envelopment with the consequence that virus spread is restricted to a cell-associated pathway."

[https://www.ncbi.nlm.nih.gov/pubmed/20181688]

MCMV mutants got generated that express with tdTomato, eGFP or mCherry Icon

MCMV mutants got generated that express with tdTomato, eGFP or mCherry

MCMV mutants, tdTomato, Cherry, eGFP fibroplasts

GFP immunofluorescence on HEK 293 cells Icon

GFP immunofluorescence on HEK 293 cells

"Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture."

[https://en.wikipedia.org/wiki/HEK_293_cells]

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp) Icon

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp)

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis."

[https://en.wikipedia.org/wiki/Natural_killer_cell]

NK92 cells natural killer cells (human cell line) same samples microwell plate. Icon

NK92 cells natural killer cells (human cell line) same samples microwell plate.

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis."

[https://en.wikipedia.org/wiki/Natural_killer_cell]

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse cd45 stain Icon

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse cd45 stain

"The spleen is an organ found in virtually all vertebrates. Similar in structure to a large lymph node, it acts primarily as a blood filter. A study published in 2009 using mice found that the red pulp of the spleen forms a reservoir that contains over half of the body's monocytes." [https://en.wikipedia.org/wiki/Spleen]

Foci assay: total well view Icon

Foci assay: total well view

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed."

[https://en.wikipedia.org/wiki/Virus_quantification]

Foci assay: Very large Foci Icon

Foci assay: Very large Foci

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed."

[https://en.wikipedia.org/wiki/Virus_quantification]

GFP/DAPI-expressed virus infected Icon

GFP/DAPI-expressed virus infected

"Using DNA recombinant technology, scientists combine the GFP gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell."

[https://embryo.asu.edu/pages/green-fluorescent-protein]

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