BIOREADER®-7000-F z i micro

Versatile, Inverted high resolution Zoom
Fluoro/VISReader, micro+macromode with innovative power LED fluoro excitation.

BIOREADER®-7000-F z i micro
Versatile, Inverted high resolution Zoom
Fluoro/VISReader, micro+macromode with innovative power LED fluoro excitation.

Flexibility

The Bioreader® hardware may be upgraded on site. Brickstone principle.

Independence

Bioreader® operates with all commonly used Elispot/Fluorospot/Plaque/Fluoro-Plaque/CFU assays.
You can choose among all major assay manufactures.

Features
  • Front loader, automatic door
  • Dual tele-centric illumination
  • Loudspeaker and interactive training program
  • Excel/Word/Power Point and LIMS export capabilities
  • 'routine' and 'research' user interface
  • High contrast/low noise Cameras 5-41 MPixel available
Software

biocreader_7000_fzi_micro_software.png

Eazyreader® software

  • Innovative Eazyreader® software combines ‘easy of use’ and versatility and flexibility
  • Optimized applications for commonly used operations like ‘routine scan’
  • QC presents only the icons you requires for this job
  • Scan, analyze and overlay live time
  • creates up to 7 images for each well simultaneously during te scan already
  • ‘Profiling’ help to create ‘user independent’ measure protocols for Elispot beginners and references fro experts
  • Verified in collaborative studies
  • Export options including reports with all scalable images and results in one file
  • Customer specific report templates
  • Video clips and content specific help files
  • Qualified installation and training on side with each Bioreader®
  • On side support and internet remote support
  • ‘Classified’ measure protocols ‘history’ tracking and comparison tools
  • User specific projects, designs, protocols and studies prevent from mixup
  • More accurate ‘cytokine quantification’ based on the patented ‘photometric’ dual illumination system
  • CE EMV certificate
  • 21 cfr part 11 module
  • Full automation optional with ‘feeding system’

 

Dimension

width
60 cm
height
80 cm
depth
45 cm

Weight

weight
40 kg
The BIOREADER®-7000-F z i micro can also use with the follow applications:
Cell compartment visualization and cell transfection efficiency Icon

Cell compartment visualization and cell transfection efficiency

The Bioreader® 7000-F-z with micro-resolution can determine the transfection rate. If compartments are transfected with specific proteins and labelled with individual fluorophores they can be differciated.

Elispot in 384 multifilterplates Icon

Elispot in 384 multifilterplates

Small volume, high throughput encymatic Elispot.

"The enzyme-linked immune absorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell."

[https://en.wikipedia.org/wiki/ELISpot]

Elispot single enzymatic Icon

Elispot single enzymatic

Blue/red/green/silver substrat Elispot. Detection antibody, enzyme-conjugate and precipitating substrate.

Infection assay Icon

Infection assay

Variable microscopic magnification.

Green fluorescence.

Adherent cells (primary human fibroblasts) in 96-well micro plates. Fluorophores: DAPI and AlexaFluor488.

Fluorospot 1-4 colors Icon

Fluorospot 1-4 colors

"The FluoroSpot measures the secretion of several cytokines by the same cell using a cytokine-specific fluorochromic antibody studied."

[https://fr.wikipedia.org/wiki/FluoroSpot]

RFP-expressed virus infected Icon

RFP-expressed virus infected

"Using DNA recombinant technology, scientists combine the GFP gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell."

[https://embryo.asu.edu/pages/green-fluorescent-protein but with RFP instead of GFP]

GFP/DAPI-expressed virus infected Icon

GFP/DAPI-expressed virus infected

"Using DNA recombinant technology, scientists combine the GFP gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell."

[https://embryo.asu.edu/pages/green-fluorescent-protein]

Unstained cells Icon

Unstained cells

Alveolar epithelial cells (AEC) Icon

Alveolar epithelial cells (AEC)

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

AEC graylevels Icon

AEC graylevels

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

AEC color Icon

AEC color

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

Dual Elispot enzymatic 1-2 colors Icon

Dual Elispot enzymatic 1-2 colors

blue/red substrate and mixed color violet

Cell viability Icon

Cell viability

6-CF appears as green fluorescence.

Live/dead Icon

Live/dead

Live cells are white.

Dead cells are black.

Tryphaneblue stain.

Transgene assay Icon

Transgene assay

"In its most precise usage, the term transgene describes a segment of DNA containing a gene sequence that has been isolated from one organism and is introduced into a different organism."

[https://en.wikipedia.org/wiki/Transgene]

Cells transfected with an expression plasmid encoding eGFP.

Live single cells Icon

Live single cells

Bright field application Jurkatcells.

Live/apoptotic cells Icon

Live/apoptotic cells

NUCblue/interference/NUCgreen.

Mix of Jurkatcells untreated cell proliferate and TNF/CHX-treated that causes to apoptosis.

MCMV (Murine Cytomegalovirus) greenfluoro remission Icon

MCMV (Murine Cytomegalovirus) greenfluoro remission

"Cytomegalovirus (CMV) (from the Greek cyto-, "cell," and megalo-, "large") is a genus of viruses in the order Herpesvirales."

[https://en.wikipedia.org/wiki/Cytomegalovirus]

MCMV (Murine Cytomegalovirus) red fluoro remission. Icon

MCMV (Murine Cytomegalovirus) red fluoro remission.

"Cytomegalovirus (CMV) (from the Greek cyto-, "cell," and megalo-, "large") is a genus of viruses in the order Herpesvirales."

[https://en.wikipedia.org/wiki/Cytomegalovirus]

MCMV (Mouse Cytomegalovirus) unstained Icon

MCMV (Mouse Cytomegalovirus) unstained

HCMV (Human cytomegalovirus): unstained Icon

HCMV (Human cytomegalovirus): unstained

"The glycoprotein gO (UL74) of human cytomegalovirus (HCMV) forms a complex with gH/gL. Virus mutants with a deletion of gO show a defect in secondary envelopment with the consequence that virus spread is restricted to a cell-associated pathway."

[https://www.ncbi.nlm.nih.gov/pubmed/20181688]

MCMV mutants got generated that express with tdTomato, eGFP or mCherry Icon

MCMV mutants got generated that express with tdTomato, eGFP or mCherry

MCMV mutants, tdTomato, Cherry, eGFP fibroplasts

GFP immunofluorescence on HEK 293 cells Icon

GFP immunofluorescence on HEK 293 cells

"Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture."

[https://en.wikipedia.org/wiki/HEK_293_cells]

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp) Icon

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp)

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis."

[https://en.wikipedia.org/wiki/Natural_killer_cell]

NK92 cells natural killer cells (human cell line) same samples microwell plate. Icon

NK92 cells natural killer cells (human cell line) same samples microwell plate.

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis."

[https://en.wikipedia.org/wiki/Natural_killer_cell]

Foci assay: total well view Icon

Foci assay: total well view

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed."

[https://en.wikipedia.org/wiki/Virus_quantification]

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse cd45 stain Icon

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse cd45 stain

"The spleen is an organ found in virtually all vertebrates. Similar in structure to a large lymph node, it acts primarily as a blood filter. A study published in 2009 using mice found that the red pulp of the spleen forms a reservoir that contains over half of the body's monocytes." [https://en.wikipedia.org/wiki/Spleen]

Foci assay: Very large Foci Icon

Foci assay: Very large Foci

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed."

[https://en.wikipedia.org/wiki/Virus_quantification]

Plaque assay extremely large spots Icon

Plaque assay extremely large spots

"The TCID50 (Median Tissue Culture Infectious Dose) is one of the methods used when verifying viral titer. TCID50 signifies the concentration at which 50% of the cells are infected when a test tube or well plate upon which cells have been cultured is inoculated with a diluted solution of viral fluid."

[https://www.zeomic.co.jp/en/glossary/virus/71]

TCID50 viral assay Icon

TCID50 viral assay

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

CV Plaque assay.Example with large and fuzzy plaques Icon

CV Plaque assay.Example with large and fuzzy plaques

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

Plaque assay: very bright plaques in 24 well plate Icon

Plaque assay: very bright plaques in 24 well plate

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

Neutralization assay Icon

Neutralization assay

"The plaque reduction neutralization test is used to quantify the titer of neutralizing antibodies for a virus. The serum sample or solution of antibodies to be tested is diluted and mixed with a viral suspension."

[https://en.wikipedia.org/wiki/Plaque_reduction_neutralization_test]

Neutralization assay: accepted plaques are surrounded Icon

Neutralization assay: accepted plaques are surrounded

"The plaque reduction neutralization test is used to quantify the titer of neutralizing antibodies for a virus. The serum sample or solution of antibodies to be tested is diluted and mixed with a viral suspension."

[https://en.wikipedia.org/wiki/Plaque_reduction_neutralization_test]

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 568 labelled secondary antibodies Icon

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 568 labelled secondary antibodies

"Marek's disease is a highly contagious viral neoplastic disease in chickens. The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes. Viruses related to MDV appear to be benign and can be used as vaccine strains to prevent Marek's disease."

[https://en.wikipedia.org/wiki/Marek's_disease]

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies Icon

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies

"Marek's disease is a highly contagious viral neoplastic disease in chickens. The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes. Viruses related to MDV appear to be benign and can be used as vaccine strains to prevent Marek's disease."

[https://en.wikipedia.org/wiki/Marek's_disease]

Organoids Icon

Organoids

"An organoid is a miniaturized and simplified version of an organ produced in vitro in three dimensions that shows realistic micro-anatomy. The technique for growing organoids has rapidly improved since the early 2010s."

[https://en.wikipedia.org/wiki/Organoid]

Dual Transgene assay Icon

Dual Transgene assay

Transgen:

A segment of DNA introduced to some other organism.

See [https://en.wikipedia.org/wiki/Transgene]

Various colonies in 6 well plates and on 35 mm - 100 mm petridishes Icon

Various colonies in 6 well plates and on 35 mm - 100 mm petridishes

Various media/strain combinations on petridishes Icon

Various media/strain combinations on petridishes

Antibiotic potency USP/PhEur/DIN/BP Petrishes Icon

Antibiotic potency USP/PhEur/DIN/BP Petrishes

The antibiotic potency assay measures the effectiveness of an antibiotic by the degree of growth inhibition on susceptible strains of microorganisms at different concentrations.

Adenovirus Icon

Adenovirus

"Adenoviruses (members of the family Adenoviridae) are medium-sized (90–100 nm), nonenveloped (without an outer lipid bilayer) viruses with an icosahedral nucleocapsid containing a double stranded DNA genome.

Their name derives from their initial isolation from human adenoids in 1953.

They have a broad range of vertebrate hosts; in humans, more than 50 distinct adenoviral serotypes have been found to cause a wide range of illnesses, from mild respiratory infections in young children (known as the common cold) to life-threatening multi-organ disease in people with a weakened immune system."

[https://en.wikipedia.org/wiki/Adenoviridae]

Single cell infection assay Icon

Single cell infection assay

Hochauflösender Virus-infections-assay Analysator.

Entwicklung Anti-Viraler Assays

Z.B. für CORVID,Hepatitis,Herpes,Adeno,ZIKA,Marek oder Cytomegalovirus.

Immunhistochemische oder Fluorophore oder Protein Färbungen. 

Z.B. Leber,Milz oder Lungen Zellen.Epitel,Vero,HEK.

Einzelzellen,Foci oder Plaques.

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