BIOREADER®-7000-F z i micro

Versatile, Inverted high resolution Zoom
Fluoro/VIS Reader, micro+macromode with innovative power LED fluoro excitation.

BIOREADER®-7000-F z i micro
Versatile, Inverted high resolution Zoom
Fluoro/VIS Reader, micro+macromode with innovative power LED fluoro excitation.

Flexibility

The Bioreader® hardware may be upgraded on site. Brickstone principle.

Independence

Bioreader® operates with all commonly used Elispot/Fluorospot/Plaque/Fluoro-Plaque/CFU assays.
You can choose among all major assay manufactures.

This Bioreader®-7000-F z i micro provides an automatic zoom range for full field of view 35 mm -100 mm Petridish, 6 - 384 well plates  and 1 mm field of view microscopic slide or hemocytometer.

Providing a resolution for good shape recognition with single cell > 3 µ, Foci 10-2000 µ or Plaques 100-5000 µ, cell clones 50 - 2000 µ and more.

Model variants    Fluor  lightsources   Remission filters installed      VIS lightsources

Alpha                                           1                                             1                       3

Beta                                             2                                             2                       3

Gamma                                       3                                             3                       3

Delta                                            6                                             6-8                    3

In vitro antiviral vaccine analyser

This Bioreader ® supports testing and development of  antiviral vaccines.

Example#1: The automatic analyses of   Plaque reductions assay.

This assay applies diluted doses of a -possibly- antiviral drug into microwell plates. The Bioreader automatically observes its capability to suppress the formation of plaques(-holes-) in the bacterial lawn.

The ‘Plaque reductions neutralization test’ (PRNT) is a variation of this test. It is an attempt to find an antibody that neutralizes a specific virus. This is a very valuable tool in antiviral vaccine research.

Examplel#2: The automatically evaluation of the Yield reduction assay

In several steps of operation cell are infected in presence of variable doses of a possibly antiviral drug substance.

Thereafter the virus titer is determined in the liquid by use of the plaque or TCID50 assay with of  the Bioreader®.

The titer is calculated as the quotient of the positive cells/all cells, taking in account the virus dilution factor and the volume of the inoculum.

Features
  • Front loader, automatic door
  • Dual tele-centric illumination
  • Loudspeaker and interactive training program
  • Excel/Word/Power Point and LIMS export capabilities
  • 'routine' and 'research' user interface
  • High contrast/low noise Cameras 5-41 MPixel available
Software

biocreader_7000_fzi_micro_software.png

Eazyreader® software

  • Innovative Eazyreader® software combines ‘easy of use’ and versatility and flexibility
  • Optimized applications for commonly used operations like ‘routine scan’
  • QC presents only the icons you requires for this job
  • Scan, analyze and overlay live time
  • creates up to 7 images for each well simultaneously during te scan already
  • ‘Profiling’ help to create ‘user independent’ measure protocols for Elispot beginners and references fro experts
  • Verified in collaborative studies
  • Export options including reports with all scalable images and results in one file
  • Customer specific report templates
  • Video clips and content specific help files
  • Qualified installation and training on side with each Bioreader®
  • On side support and internet remote support
  • ‘Classified’ measure protocols ‘history’ tracking and comparison tools
  • User specific projects, designs, protocols and studies prevent from mixup
  • More accurate ‘cytokine quantification’ based on the patented ‘photometric’ dual illumination system
  • CE EMV certificate
  • 21 cfr part 11 module
  • Full automation optional with ‘feeding system’

 

Dimension

width
60 cm
height
80 cm
depth
45 cm

Weight

weight
40 kg
The BIOREADER®-7000-F z i micro can also use with the follow applications:
Cell compartment visualization and cell transfection efficiency Icon

Cell compartment visualization and cell transfection efficiency

The Bioreader® 7000-F-z with micro-resolution can determine the transfection rate. If compartments are transfected with specific proteins and labelled with individual fluorophores they can be differciated.

Elispot in 384 multifilterplates Icon

Elispot in 384 multifilterplates

Small volume, high throughput encymatic Elispot.

"The enzyme-linked immune absorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell."

Why Elispot?

•The cytokine Elispot assay is designed to enumerate cytokine secreting cells in single cell suspensions of lymphoid tissue, CNS tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMC).

•The assay has the advantage of detecting only activated/memory T cells and the cytokine release can be detected at the single cell level, allowing direct determination of T cell frequencies.

The ELISPOT assay is an effective tool to enumerate antigen-specific T cells in the circulation of immunized humans and animals at much lower frequencies than possible with other currently available methods.

The ELISPOT assay has proven to be a sensitive and unique system to follow disease progression in human individuals or animals. Several studies have indicated that alterations in the frequency of cytokine pc in different compartments of the body adequately reflect changes in immune function.

The ELISPOT assay may be used to determine effects of drugs, chemicals or other compounds on cytokine secretion in vitro, thereby providing data on their putative modulatory effects on immune function in vivo

The ELISPOT assay is currently being used increasingly for the quantitative assessment of peptide reactive T lymphocytes from PBMC in infectious disease (3, 9) or in the course of vaccination trials aimed at the induction of tumor-specific T cells in patients with cancer.

Elispot may be used for Diagnosis of genetic defects,Allergic diseases,Autoimmune diseases,Transplantation,Cancer research,Acute inflammation,Acute infectious diseases and septic shock.•

[https://en.wikipedia.org/wiki/ELISpot]

Elispot single enzymatic Icon

Elispot single enzymatic

Blue/red/green/silver substrat Elispot. Detection antibody, enzyme-conjugate and precipitating substrate.

 

Infection assay Icon

Infection assay

GFP and Alexa 488 fluoro remission

Variable microscopic magnification.

Green fluorescence.

Adherent cells (primary human fibroblasts) in 96-well micro plates. Fluorophores: DAPI and AlexaFluor488.

Fluorospot 1-4 colors Icon

Fluorospot 1-4 colors

"The FluoroSpot measures the secretion of several cytokines by the same cell using a cytokine-specific fluorochromic antibody studied."

Why use Fluorospot

•Enzymatic Elispot labeling
•allows to label 1-2 cytokines per cell synchronous.
•If you are interested to see ‘double secreting’ cells the stain of two cells in neighborhood may overlap. So, It is not clear if two cells are overlapping or one cells is really secreting two different cytokines ( see below left)

‘Fluorospot’ labeling
Allows to label a (theoretical unlimited number different cytokines) Because excitation for each cytokine is applied -one after the other-, decisions for double secreting cells is clearly.
One Image is acquired for each cytokine!

Currently Fluorospot assays with 1-4 Fluorophores are on the market.

Fluorospot offer's significant advantages over colorimetric formats, particularly in the areas of multiplexing and automated spot detection.

Moreover, as spot development is not enzymatic, signal intensity is directly proportional to the amount of analyte within.

[https://fr.wikipedia.org/wiki/FluoroSpot]

RFP-expressed virus infected Icon

RFP-expressed virus infected

"Using DNA recombinant technology, scientists combine the GFP gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell."

[https://embryo.asu.edu/pages/green-fluorescent-protein but with RFP instead of GFP]

GFP/DAPI-expressed virus infected Icon

GFP/DAPI-expressed virus infected

"Using DNA recombinant technology, scientists combine the GFP gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell."

[https://embryo.asu.edu/pages/green-fluorescent-protein]

Alveolar epithelial cells (AEC) Icon

Alveolar epithelial cells (AEC)

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

Unstained cells Icon

Unstained cells

AEC color Icon

AEC color

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

AEC graylevels Icon

AEC graylevels

"Typically, type 1 alveolar cells comprise the major gas exchange surface of the alveolus and are integral to the maintenance of the permeability barrier function of the alveolar membrane. Type 2 pneumocytesare the progenitors of type 1 cells and are responsible for surfactant production and homeostasis."

[https://www.sciencedirect.com/topics/medicine-and-dentistry/alveolar-type-i-cells]

The alveolar epithelium is a major target in toxic exposures of the lung because of its structural delicacy and proximity to inhaled toxicants. Type II epithelial cells are important in maintaining the integrity of alveolar epithelium and normal lung function.

Dual Elispot enzymatic 1-2 colors Icon

Dual Elispot enzymatic 1-2 colors

blue/red substrate and mixed color violet

Live/dead Icon

Live/dead

Live cells are white.

Dead cells are black.

Tryphaneblue stain.

Cell viability Icon

Cell viability

6-CF appears as green fluorescence.

Live single cells Icon

Live single cells

Bright field application Jurkatcells.

Live/apoptotic cells Icon

Live/apoptotic cells

NUCblue/interference/NUCgreen.

Mix of Jurkatcells untreated cell proliferate and TNF/CHX-treated that causes to apoptosis.

Transgene assay Icon

Transgene assay

"In its most precise usage, the term transgene describes a segment of DNA containing a gene sequence that has been isolated from one organism and is introduced into a different organism."

[https://en.wikipedia.org/wiki/Transgene]

Cells transfected with an expression plasmid encoding eGFP.

MCMV (Murine Cytomegalovirus) greenfluoro remission Icon

MCMV (Murine Cytomegalovirus) greenfluoro remission

"Cytomegalovirus (CMV) (from the Greek cyto-, "cell," and megalo-, "large") is a genus of viruses in the order Herpesvirales."

[https://en.wikipedia.org/wiki/Cytomegalovirus]

MCMV (Mouse Cytomegalovirus) unstained Icon

MCMV (Mouse Cytomegalovirus) unstained

MCMV (Murine Cytomegalovirus) red fluoro remission. Icon

MCMV (Murine Cytomegalovirus) red fluoro remission.

"Cytomegalovirus (CMV) (from the Greek cyto-, "cell," and megalo-, "large") is a genus of viruses in the order Herpesvirales."

[https://en.wikipedia.org/wiki/Cytomegalovirus]

HCMV (Human cytomegalovirus): unstained Icon

HCMV (Human cytomegalovirus): unstained

"The glycoprotein gO (UL74) of human cytomegalovirus (HCMV) forms a complex with gH/gL. Virus mutants with a deletion of gO show a defect in secondary envelopment with the consequence that virus spread is restricted to a cell-associated pathway."

[https://www.ncbi.nlm.nih.gov/pubmed/20181688]

MCMV mutants got generated that express with tdTomato, eGFP or mCherry Icon

MCMV mutants got generated that express with tdTomato, eGFP or mCherry

MCMV mutants, tdTomato, Cherry, eGFP fibroplasts

GFP immunofluorescence on HEK 293 cells Icon

GFP immunofluorescence on HEK 293 cells

"Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture."

[https://en.wikipedia.org/wiki/HEK_293_cells]

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp) Icon

NK92 cells natural killer cells (human cell line) expressing green fluorescent protein (gfp)

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis."

[https://en.wikipedia.org/wiki/Natural_killer_cell]

NK92 cells natural killer cells (human cell line) same samples microwell plate. Icon

NK92 cells natural killer cells (human cell line) same samples microwell plate.

"Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. Typically, immune cells detect major histocompatibility complex (MHC) presented on infected cell surfaces, triggering cytokine release, causing lysis or apoptosis."

[https://en.wikipedia.org/wiki/Natural_killer_cell]

Foci assay: total well view Icon

Foci assay: total well view

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed."

[https://en.wikipedia.org/wiki/Virus_quantification]

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse cd45 stain Icon

Mouse spleen cells mostly lymphocytes stained with FITC-conjugated anti mouse cd45 stain

"The spleen is an organ found in virtually all vertebrates. Similar in structure to a large lymph node, it acts primarily as a blood filter. A study published in 2009 using mice found that the red pulp of the spleen forms a reservoir that contains over half of the body's monocytes." [https://en.wikipedia.org/wiki/Spleen]

Foci assay: Very large Foci Icon

Foci assay: Very large Foci

"The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed."

[https://en.wikipedia.org/wiki/Virus_quantification]

Plaque assay extremely large spots Icon

Plaque assay extremely large spots

"The TCID50 (Median Tissue Culture Infectious Dose) is one of the methods used when verifying viral titer. TCID50 signifies the concentration at which 50% of the cells are infected when a test tube or well plate upon which cells have been cultured is inoculated with a diluted solution of viral fluid."

[https://www.zeomic.co.jp/en/glossary/virus/71]

CV Plaque assay.Example with large and fuzzy plaques Icon

CV Plaque assay.Example with large and fuzzy plaques

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

TCID50 viral assay Icon

TCID50 viral assay

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

Plaque assay: very bright plaques in 24 well plate Icon

Plaque assay: very bright plaques in 24 well plate

Cells were infected and covered with an overlay. Cells stained with CV survive. Unstained cells were killed by the virus.

Neutralization assay Icon

Neutralization assay

"The plaque reduction neutralization test is used to quantify the titer of neutralizing antibodies for a virus. The serum sample or solution of antibodies to be tested is diluted and mixed with a viral suspension."

[https://en.wikipedia.org/wiki/Plaque_reduction_neutralization_test]

Neutralization assay: accepted plaques are surrounded Icon

Neutralization assay: accepted plaques are surrounded

"The plaque reduction neutralization test is used to quantify the titer of neutralizing antibodies for a virus. The serum sample or solution of antibodies to be tested is diluted and mixed with a viral suspension."

[https://en.wikipedia.org/wiki/Plaque_reduction_neutralization_test]

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies Icon

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 488 labelled secondary antibodies

"Marek's disease is a highly contagious viral neoplastic disease in chickens. The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes. Viruses related to MDV appear to be benign and can be used as vaccine strains to prevent Marek's disease."

[https://en.wikipedia.org/wiki/Marek's_disease]

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 568 labelled secondary antibodies Icon

Detection of Marek's disease virus Plaques after staining with specific anti-MDV antibodies and Alexa 568 labelled secondary antibodies

"Marek's disease is a highly contagious viral neoplastic disease in chickens. The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes. Viruses related to MDV appear to be benign and can be used as vaccine strains to prevent Marek's disease."

[https://en.wikipedia.org/wiki/Marek's_disease]

Dual Transgene assay Icon

Dual Transgene assay

Transgen:

A segment of DNA introduced to some other organism.

See [https://en.wikipedia.org/wiki/Transgene]

Organoids Icon

Organoids

"An organoid is a miniaturized and simplified version of an organ produced in vitro in three dimensions that shows realistic micro-anatomy. The technique for growing organoids has rapidly improved since the early 2010s."

[https://en.wikipedia.org/wiki/Organoid]

Various colonies in 6 well plates and on 35 mm - 100 mm petridishes Icon

Various colonies in 6 well plates and on 35 mm - 100 mm petridishes

Various media/strain combinations on petridishes Icon

Various media/strain combinations on petridishes

Antibiotic potency USP/PhEur/DIN/BP Petrishes Icon

Antibiotic potency USP/PhEur/DIN/BP Petrishes

The antibiotic potency assay measures the effectiveness of an antibiotic by the degree of growth inhibition on susceptible strains of microorganisms at different concentrations.

Adenovirus Icon

Adenovirus

"Adenoviruses (members of the family Adenoviridae) are medium-sized (90–100 nm), nonenveloped (without an outer lipid bilayer) viruses with an icosahedral nucleocapsid containing a double stranded DNA genome.

Their name derives from their initial isolation from human adenoids in 1953.

They have a broad range of vertebrate hosts; in humans, more than 50 distinct adenoviral serotypes have been found to cause a wide range of illnesses, from mild respiratory infections in young children (known as the common cold) to life-threatening multi-organ disease in people with a weakened immune system."

[https://en.wikipedia.org/wiki/Adenoviridae]

Single cell infection assay Icon

Single cell infection assay

High Resolution Virus-infections-assay Analyzer.

Development of antiviral vaccines and substances.

For instance for CORVID, Hepatitis, Herpes, Adeno, ZIKA, Marek or Cytomegalovirus virus and more.

Immunohistochemical and Fluorophore or protein staining. 

Liver, spleen or lung cells, Vero (kidney epithelial, HEK(Human Embryonic Kidney) and more.

Single cell > 3 µ,Foci  10-2000 µ or Plaques 100-5000 µ,cell clones 50 - 2000 µ and more

COV-2 Icon

COV-2

Vero cells infected by SARS-CoV-2

Inverted Immune fluorescence test
Secondary Antibody Cy3 AffiniPure Goat Anti-Human IgG

SARS_COV_2 Icon

SARS_COV_2

Vero cells infected by SARS-CoV-2

Inverted Immune fluorescence test
Secondary Antibody Cy3 AffiniPure Goat Anti-Human IgG

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