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Background: Reading of agardiffusion tests is currently performed mainly visually, except for inhibition of zones generated by paper discs, test tablets or wells cut into the agar lawn for which several automatic systems are available (e.g. BIOCOUNT). Theses systems are based on image processing principles such as image detection, image smoothening, edge detection, edge enhancing, and area detection. However, all these systems determine and report only the idealized area of the inhibition zone, respectively report the diameter of these almost round (symmetric) inhibition zones. Aim of this study was to determine if asymmetric (e.g. elliptic or irregularly shaped) inhibition zones can be detected reliably and the included inhibition zone measured automatically. In addition, it was investigated whether from asymmetric inhibition zones of antifungal agents reproducible endpoints (e.g. MIC’s from strip-tests) could be derived. |
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A study by: A.F. Schmalreck, K. Becker, W. Fegeler |
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